Urine samples of seropositive patients with normal kidney function were tested for NY-ESO-1 antibody by Western blotting and enzyme-linked immunosorbent assay (ELISA). Antibodies to NY-ESO-1 were found in the urine of patients whose NY-ESO-1 serum antibody titers

NY-ESO-1 is one of the most immunogenic tumor antigens known to date. Spontaneous humoral and cellular immune responses against NY-ESO-1 are detected in a substantial proportion of patients with NY-ESO-1 positive cancers. NY-ESO-1 serum antibody is dependent on the presence of NY-ESO-1+ cancer cells, and antibody titers correlate with the clinical development of the disease. NYESO-1 serum antibody is associated with detectable NY-ESO-1-specific CD8+ T cell reactivity. High titers of NY-ESO-1 serum antibodies are found in patients with advanced NY-ESO-1+ malignancies. Urine samples of seropositive patients with normal kidney function were tested for NY-ESO-1 antibody by Western blotting and enzyme-linked immunosorbent assay (ELISA). Antibodies to NY-ESO-1 were found in the urine of patients whose NY-ESO-1 serum antibody titers were 1:10,000 or higher by Western blotting. In patients with weak (positive at 1:250, negative at 1:1,000) or no reactivity, urine antibody was not detectable. No urine NY-ESO-1 antibody was found in patients without detectable NY-ESO-1 serum antibody. Our results show that urine analysis for NY-ESO-1 antibody identifies patients with strong NY-ESO-1 immunity. Urine antibody detection may also be of value in the monitoring of spontaneous and vaccine-induced immunity against other defined tumor antigens. Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-113616 Published Version Originally published at: Jäger, Dirk; Stockert, Elisabeth; Karbach, Julia; Herrlinger, Kristina; Atmaca, Akin; Arand, Michael; Chen, Yao-Tseng; Gnjatic, Sacha; Old, Lloyd J; Knuth, Alexander; Jäger, Elke (2002). Urine antibody against human cancer antigen NY-ESO-1. Cancer Immunity, 2:10. Cancer Immunity 2:10 (2002) ARTICLE LATEST PAPERS SEARCH for PAPERS Printer-friendly PDF Comment(s) >Abstract >Introduction >Results >Discussion >References >Materials & methods >Contact authors Cancer Immunity, Vol. 2, p. 10 (6 August 2002) Submitted: 17 June 2002. Accepted: 24 July 2002. Contributed by: A Knuth Urine antibody against human cancer antigen NY-ESO-1 Dirk Jäger1, Elisabeth Stockert2, Julia Karbach1, Kristina Herrlinger1, Akin Atmaca1, Michael Arand3, YaoTseng Chen2, Sacha Gnjatic2, Lloyd J. Old2, Alexander Knuth1, and Elke Jäger1 1II. Medizinische Klinik, Hämatologie Onkologie, Krankenhaus Nordwest, Frankfurt, Germany 2Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York, USA 3Institut für Toxikologie, Johannes Gutenberg Universität, Mainz, Germany